Journal: Scientific Reports

Article Title: Direct Visualization of De novo Lipogenesis in Single Living Cells

doi: 10.1038/srep06807

Figure Lengend Snippet: (a). Glucose-d 7 derived lipogenesis were measured in prostate cell lines, including normal epithelial RWPE1 cell and cancerous LNCaP, PC3 cells by SRS imaging at C–D and C–H vibration. (b). Quantitative analysis of de-novo lipogenesis level in prostate cell lines. Data were shown as mean + SD. N ≥ 10 for each group. *** indicates p < 0.001 by student T test. (c). SRS imaging of prostate cells and (d). pancreatic cells treated with 100 μM palmitic acid-d 31 for 24 hours.

Article Snippet: Glucose-d 7 , glutamine-d 5 and palmitic acid-d 31 were purchased from Cambridge Isotope Laboratories, Inc. Fatty acid synthase (FAS) inhibitor C75 was purchased from Cayman Chemical.

Techniques: Derivative Assay, Imaging

Journal: Macromolecular Rapid Communications

Article Title: Online Coupling of 80 MHz Benchtop 2D‐COSY NMR to HPLC

doi: 10.1002/marc.202500239

Figure Lengend Snippet: HPLC‐benchtop 2D‐NMR analysis with stop‐flow for a mixture of methyl paraben MP and propyl paraben PP (50 g L −1 each in acetone/water 60/40 v/v): 1 H NMR elugram and corresponding flow rate profile (a), COSY spectra (b–e). The 1 H NMR elugrams and corresponding flow rate profile (a) show the stop‐flow increments in the indirect dimension with a 1 min ramp down, a 3 min stop, and 1 min ramp back up. COSY spectra (at Ernst condition, with 1 scan, 256 increments in the indirect dimension, 37.5% NUS density) recorded in 1 min 54 s during the stops in MP peak (b, c) and PP peak (d, e) are shown covering the aromatic region (b, d), and the aliphatic region (c, e, with acetone signal cut out for display through an axis break at 2.25–2.70 ppm). See Figure (Supporting Information) for COSY spectrum recorded in the baseline BL. * indicates an acetone signal 13 C satellite and # an intermodulation artifact.

Article Snippet: Methyl paraben (methyl‐4‐hydroxybenzoate, 99%) was sourced from Thermo Scientific (Kandel, Germany), propyl paraben (propyl‐4‐hydroxybenzoate, 99+%) from ACROS (Geel, Belgium), acetone (≥99.8%, HPLC grade) and water (HPLC gradient grade) from Fisher Scientific (Schwerte, Germany).

Techniques:

Journal: Macromolecular Rapid Communications

Article Title: Online Coupling of 80 MHz Benchtop 2D‐COSY NMR to HPLC

doi: 10.1002/marc.202500239

Figure Lengend Snippet: HPLC‐benchtop 2D‐NMR analysis with slow‐flow for a mixture of methyl paraben MP and propyl paraben PP (50 g L −1 each in acetone/water 60/40 v/v): UV absorption (325 nm) elugram and corresponding flow rate profile (a), COSY spectra (b–e). The UV absorption elugram and corresponding flow rate profile (a) show the position of the COSY spectra recording (blue lines). COSY spectra (at Ernst condition, with 1 scan, 256 increments in the indirect dimension, 37.5% NUS density) were recorded in 1 min 54 s at slow‐flow (0.4 mL min −1 ) in MP peak (b, c) and in PP peak (d, e). COSY spectra are shown covering the aromatic region (b, d), and the aliphatic region (c, e, with acetone signal cut out for display through an axis break at 2.25–2.70 ppm). See Figure (Supporting Information) for COSY spectrum recorded in the baseline BL. * indicates an acetone signal 13 C satellite and # an intermodulation artifact.

Article Snippet: Methyl paraben (methyl‐4‐hydroxybenzoate, 99%) was sourced from Thermo Scientific (Kandel, Germany), propyl paraben (propyl‐4‐hydroxybenzoate, 99+%) from ACROS (Geel, Belgium), acetone (≥99.8%, HPLC grade) and water (HPLC gradient grade) from Fisher Scientific (Schwerte, Germany).

Techniques:

Journal: Macromolecular Rapid Communications

Article Title: Online Coupling of 80 MHz Benchtop 2D‐COSY NMR to HPLC

doi: 10.1002/marc.202500239

Figure Lengend Snippet: HPLC‐benchtop 2D‐NMR analysis in steady‐flow mode for a mixture of methyl paraben MP and propyl paraben PP (50 g L −1 each in acetone/water 60/40 v/v): 1 H NMR elugram and corresponding flow rate profile (a), COSY spectra (b–e). The 1 H NMR elugram and corresponding flow rate profile (a) show the position of the COSY spectra recording (blue lines). COSY spectra (at Ernst condition, with 1 scan, 256 increments in the indirect dimension, 37.5% NUS density) recorded in 1 min 54 s during the MP peak (b, c) and PP peak (d, e) are shown covering the aromatic region (b, d), and the aliphatic region (c, e, with acetone signal cut out for display through an axis break at 2.25–2.70 ppm). See Figure (Supporting Information) for COSY spectrum recorded in the baseline BL. * indicates an acetone signal 13 C satellite and # an intermodulation artifact.

Article Snippet: Methyl paraben (methyl‐4‐hydroxybenzoate, 99%) was sourced from Thermo Scientific (Kandel, Germany), propyl paraben (propyl‐4‐hydroxybenzoate, 99+%) from ACROS (Geel, Belgium), acetone (≥99.8%, HPLC grade) and water (HPLC gradient grade) from Fisher Scientific (Schwerte, Germany).

Techniques:

Journal: Macromolecular Rapid Communications

Article Title: Online Coupling of 80 MHz Benchtop 2D‐COSY NMR to HPLC

doi: 10.1002/marc.202500239

Figure Lengend Snippet: Signal‐to‐noise ratio for the cross‐peak at 8.05,7.20 ppm in the COSY spectra of methyl paraben (MP) and propyl paraben (PP) recorded with HPLC‐benchtop 2D‐NMR in stop‐flow, slow‐flow, and steady‐flow modes. See Figures , , and experimental section for experimental conditions (including: semi‐preparative C18 column, 1 mL injection volume, 50 g L −1 of each paraben).

Article Snippet: Methyl paraben (methyl‐4‐hydroxybenzoate, 99%) was sourced from Thermo Scientific (Kandel, Germany), propyl paraben (propyl‐4‐hydroxybenzoate, 99+%) from ACROS (Geel, Belgium), acetone (≥99.8%, HPLC grade) and water (HPLC gradient grade) from Fisher Scientific (Schwerte, Germany).

Techniques: Injection